🔬 Tissue Culture Production

Greenhouses That
Scale TC
Production.

From deflasking acclimatisation rooms to mass-hardening bays — built for tissue culture plants, powered by AI. Every batch tracked. Every parameter automated.

29Greenhouses built
98%Avg survival rate
5TC stages managed
AIPhyto Care agent
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Deflask Room95% RH
Stage I82% RH
Stage II70% RH ↓
Open BayAmbient
LIVE
phyto-care / batch-tracker / live
12 active batches
Batch IDSpeciesStageSurvivalCont.
TC-2026-047Musa nanaStage I
98%		None
TC-2026-051Dendrobium sp.Deflask
100%		None
TC-2026-038Dioscorea sp.Stage II
76%		⚠ Low
TC-2026-029Vanilla planifoliaHardening
93%		None
TC-2026-031Gerbera hybridDispatch ✓
97%		Cleared
1
Deflask Room
RH: 90–95%
2
Stage I Accli.
RH: 80–90%
3
Stage II Harden
RH: 65–80%
4
Open Hardening
Ambient
5
Dispatch Ready
Phyto Care ✓
Phyto Care Platform

Batch Intelligence. Every Flask. Every Plant.

Phyto Care tracks every TC batch from the moment it leaves the lab — humidity automation, contamination logging, AI risk scoring, and dispatch records in one platform.

💧 Humidity Automation — Step-Down
Auto-managed per batch
TC-2026-047 · Musa nana · 2,840 plants Day 14 / ~42
Deflask
Stage I
Stage II
Open
Done
95% ✓
82% ●
65%
Amb.
—
TC-2026-038 · Dioscorea · 4,120 plants Day 28 / ~42
Deflask
Stage I
Stage II
Open
Done
95% ✓
82% ✓
70% ●
Amb.
—
TC-2026-029 · Vanilla planifolia · 1,650 plants Day 35 / ~42
Deflask
Stage I
Stage II
Open
Done
95% ✓
82% ✓
65% ✓
Amb. ●
—
🔬
Ravana AI
TC Production Specialist
Online
"Batch TC-2026-038 survival dropped from 91% to 76% in 48 hours. What's happening in Stage II?"
Analysis: Rapid humidity drop + fungal stress signature
The 76% survival on TC-2026-038 correlates with the Stage II humidity dip to 63% (logged 2 days ago — below the 65% minimum for Dioscorea). Combined with temp spike to 31.2°C, this creates high VPD stress on recently-deflasked material.

Likely cause: HVAC-2B cooling coil is underperforming — I've seen this pattern twice before in this zone. Temperature drift of +1.8°C over 6 days suggests gradual degradation, not a sudden failure.

Immediate steps:
  1. Increase mist to 6min ON / 7min OFF in Stage II tonight
  2. Schedule HVAC-2B coil inspection this week
  3. Move 500 highest-stress plants to Stage I recovery bench
I've flagged a maintenance ticket. Predicted loss without action: ~18% of remaining batch.
Quality Control

Contamination Control & Zone Compliance

⚠ Contamination Incident Log
3 open
TC-2026-038
Fusarium suspected — 180 plants flagged
Isolate now
2h ago
TC-2026-044
Algae growth on tray edges — Stage I bench 3
Clean bench
Yesterday
TC-2026-047
2 plants: yellowing — possibly nutrient pH drift
Monitor
3 days ago
TC-2026-031
Cleared — final inspection passed ✓
Resolved
5 days ago
✅ Zone Compliance Scorecard
Overall: 87%
Deflask RH
97%
Stage I Temp
100%
Stage II RH
68%
Stage II Temp
74%
Open Bay RH
92%
RO Water TDS
100%
HEPA Pressure
89%
📊 Deflask Success Rate — 6 Months
Avg: 94.2%
Jan
Feb
Mar
Apr
May
Jun
≥90% — on target
<90% — flagged
⚠ Mar & Jun dips correlated with Stage II HVAC issues — AI flagged both in advance
Production Output

Dispatch Readiness & AI Risk Scoring

🚚 Dispatch Readiness Board
3 lots cleared
Batch IDPlantsSurvivalReady
TC-2026-031 1,980 97.3% Cleared ✓
TC-2026-022 3,200 95.1% Cleared ✓
TC-2026-019 840 100% Cleared ✓
TC-2026-047 2,840 98.2% Stage I · 28d
TC-2026-038 4,120 76.0% ↓ Hold — review
🤖 AI Predictive Risk Assessment
AI MODEL
TC-2026-038 at high risk of further batch failure — Stage II HVAC degradation continues
Batch TC-2026-038 · Dioscorea sp. · 4,120 plants
84% risk
Stage II misting nozzle head 3B showing reduced flow rate — possible clogging in 3–5 days
Zone: Stage II Hardening · Bay B
61% risk
Deflask room RO water TDS trending up slightly — membrane service predicted in ~18 days
Water System · Membrane Unit 1
22% risk
TC-2026-047 on track — no risk flags. All parameters within spec for 14 consecutive days.
Batch TC-2026-047 · Musa nana · 2,840 plants
4% risk
🔄 Model updated 8 minutes ago · 4 batches analysed · next scan in 22 min
TC Greenhouse Design

Built for Every Stage of TC Production.

Tissue culture plants die when acclimatisation is rushed or poorly controlled. We design facilities that get every stage right, with Phyto Care automating the transitions.

1
Deflask Room
Semi-sterile environment. High humidity (90–95%), low light, minimal airflow. Prepares plantlets for soil transition.
RH: 90–95%
2
Stage I Acclimatisation
Automated misting cycles. 80–90% RH. Controlled light increase. 7–21 days depending on species.
RH: 80–90%
3
Stage II Hardening
Stepped humidity reduction (65–80%). Shade netting. Increased photoperiod. First substrate-based water management.
RH: 65–80%
4
Open Hardening
Ambient humidity, full shade structure. Plants develop root systems. 50% shade cloth, irrigation managed.
Ambient + shade
5
Dispatch Ready
Phyto Care marks batch as cleared. Lot ID, survival rate, and origin clone recorded permanently.
Phyto Care tracked
What We Control

Every Parameter TC Plants Need.

Acclimatisation success depends on controlling the right parameters at the right time. Our builds make that automatic.

💧
Humidity
Ultrasonic foggers and misting systems with zone-by-zone RH control. Automated step-down programs take plantlets from 95% to ambient on configurable schedules.
95% → ambient staged
🌡️
Temperature
Consistent 26–30°C in acclimatisation zones. Cooling for highland TC species. Temperature uniformity across bench surfaces critical for even establishment rates.
±1°C bench uniformity
💡
Lighting
Low-intensity LED for deflask rooms, stepping up to full-spectrum in hardening bays. Programmable photoperiod for long or short-day species conditions.
1,000 → 8,000 lux staged
🌬️
Airflow
Gentle, non-turbulent air exchange prevents fungal pathogen build-up without desiccating fragile plantlets. HEPA pre-filtration for near-sterile deflask rooms.
HEPA option for Stage I
⚗️
Water Quality
RO water production for all misting and irrigation. TDS monitoring ensures supply water stays within safe limits for recently deflasked plantlets.
RO water throughout
🌱
Substrate Management
Automated sub-irrigation or capillary systems for open hardening bays. Moisture sensors per bench zone. Consistent moisture prevents root stress.
Capillary or mist
🔬
Contamination Control
Positive pressure in sensitive zones, boot wash stations, UV sterilisation options, and physical separation between contaminated and clean areas.
Zone isolation design
📡
Ravana AI
Every parameter in every zone feeds into the AI. Batch survival prediction, contamination risk scoring, maintenance forecasting — from any device, anywhere.
AI batch tracking
Any Scale

From Hobbyist Flask to Industrial TC Line.

We've built TC acclimatisation setups for individual collectors and mass-production lines handling hundreds of thousands of plants per cycle.

🏠
Home TC Setup
100–5,000 plants / cycle
  • Dedicated acclimatisation chamber
  • Mist propagation bench
  • Species-specific humidity staging
  • Phyto Care mobile monitoring
  • Budget-conscious design
Enquire →
Most Popular
🏭
Commercial TC Facility
5,000 – 25,000 sqft
  • Multi-stage zone architecture
  • Automated humidity step-down
  • Mass hardening bays
  • Full Ravana AI + batch tracking
  • Contamination control design
  • Production scheduling tools
Get a Quote →
🌏
Industrial TC Line
50,000 sqft · Multi-building
  • Master planning and feasibility
  • Multiple acclimatisation hall design
  • Industrial misting and RO systems
  • Enterprise Phyto Care deployment
  • ERP integration for dispatch
  • Maintenance service contracts
Discuss Your Project →
Track Record

29 Greenhouses. Real Plants. Real Production.

Not renders. Not proposals. Built, operational, and producing.

18Home-scale builds delivered
7Mid-range (5k–25k sqft)
4Large commercial (50k+ sqft)
100%IoT + AI connected
Our Own Facility

Forest Studio Lab, Thaiyur

Our in-house TC greenhouse and research lab in Thaiyur, Tamil Nadu. Every acclimatisation technique, zone design, and Ravana AI feature is tested at Forest Studio Lab before we deploy it to a client site. Open to serious enquiries by appointment.

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Start Your TC Greenhouse.

Tell us about your production targets — species, scale, throughput. We'll come back with a scope and budget estimate within 48 hours.

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